clonality isotype  (Developmental Studies Hybridoma Bank)

Journal: Journal of Anatomy

Article Title: The distribution of the proteoglycan FORSE‐1 in the developing mouse central nervous system

doi: 10.1111/joa.12907

Figure Lengend Snippet: List of primary antibodies used

Article Snippet: This confirmed that the FORSE‐1 antibody showed excellent immunoreactivity and a strong signal where present. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Species and clonality Isotype Manufacturers Dilution FORSE1 Mouse monoclonal IgM Developmental Studies Hybridoma Bank 1 : 200 Nestin Mouse monoclonal IgG Developmental Studies Hybridoma Bank 1 : 200 BLBP Rabbit polyclonal N/A Millipore 1 : 200 GAP‐43 Rabbit polyclonal N/A Thermo‐Fisher Scientific 1 : 500 TAG‐1 Mouse monoclonal IgM Thermo‐Fisher Scientific 1 : 200 β‐III Tubulin Mouse monoclonal IgG Thermo‐Fisher Scientific 1 : 200 Open in a separate window List of primary antibodies used Cell culture Cerebral cortex and spinal cords were dissected from E15 embryos and placed in ice‐cold calcium and magnesium‐free Hanks’ balanced salt solution (CMF HBSS) (Thermo‐Fisher Scientific, USA).

Techniques: